Coding

Part:BBa_K129002:Design

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-04)


Br-bacteriorhodopsin-light driven pump


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 95
    Illegal SpeI site found at 811
    Illegal PstI site found at 825
    Illegal NotI site found at 7
    Illegal NotI site found at 789
    Illegal NotI site found at 818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 811
    Illegal PstI site found at 825
    Illegal NgoMIV site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An additional treatment is necessary to obtain a proper conformation. The first functional expression of an archaeal rhodopsin in E. coli membranes was achieved by Shimono et al. against phoborhodopsin (sensory rhodopsin II) from Natronomonas pharaonis (ppR).


Source

LOCUS NC_010364 789 bp DNA linear BCT 12-MAR-2008 DEFINITION Halobacterium salinarum R1, complete genome. ACCESSION NC_010364 REGION: 1082241..1083029 VERSION NC_010364.1 GI:169235049 PROJECT GenomeProject:106


References